Journal: BMJ Open

Article Title: Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units

doi: 10.1136/bmjopen-2020-048187

Figure Lengend Snippet: Trial design. In the intervention arm, before the end of day 1, clinicians have to consider all the early microbiological results (mPCR FA-PPP, urine antigen assays, blood cultures and Gram stain examination of respiratory tract sample) and procalcitonin before the end of day 1, and subsequently to apply an algorithm of early antibiotics discontinuation or de-escalation (red square). This algorithm is described in . In both arms, at day 3 and day after day until day 7, clinicians are encouraged to consider antibiotic discontinuation, based on procalcitonin values and kinetics. CAP, community-acquired pneumonia; FA-PPP, FilmArray Pneumonia Panel Plus; ICU, intensive care unit; mPCR, multiplex PCR.

Article Snippet: In the experimental strategy arm, the microbiological diagnosis combines a broad-panel respiratory mPCR (FilmArray Pneumonia Panel Plus (FA-PPP), BioFire Biomérieux) with conventional microbiological investigations.

Techniques: Staining, Multiplex Assay

Journal: BMJ Open

Article Title: Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units

doi: 10.1136/bmjopen-2020-048187

Figure Lengend Snippet: Algorithm of early antibiotics de-escalation or discontinuation. The algorithm is applied in the intervention arm only (see red square, ). mPCR, multiplex PCR; ICU, intensive care unit; PCT, procalcitonin.

Article Snippet: In the experimental strategy arm, the microbiological diagnosis combines a broad-panel respiratory mPCR (FilmArray Pneumonia Panel Plus (FA-PPP), BioFire Biomérieux) with conventional microbiological investigations.

Techniques: Multiplex Assay

Journal: BMJ Open

Article Title: Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units

doi: 10.1136/bmjopen-2020-048187

Figure Lengend Snippet: Primary and secondary outcomes

Article Snippet: In the experimental strategy arm, the microbiological diagnosis combines a broad-panel respiratory mPCR (FilmArray Pneumonia Panel Plus (FA-PPP), BioFire Biomérieux) with conventional microbiological investigations.

Techniques: Bacteria, Biomarker Discovery

Journal: Frontiers in Microbiology

Article Title: A New Age in Molecular Diagnostics for Invasive Fungal Disease: Are We Ready?

doi: 10.3389/fmicb.2019.02903

Figure Lengend Snippet: List of commercially available PCR-based assays for detection of Candida spp.

Article Snippet: AusDiagnostics Pneumonia and Atypical Pneumonia panels , AusDiagnostics Pty Ltd., Mascot, NSW, Australia , Multiplex tandem PCR , Targets unknown Pneumocystis jirovecii , Cryptococcus neoformans species complex, and Aspergillus fumigatus complex.

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Sequencing, Dot Blot, Hybridization

Journal: Frontiers in Microbiology

Article Title: A New Age in Molecular Diagnostics for Invasive Fungal Disease: Are We Ready?

doi: 10.3389/fmicb.2019.02903

Figure Lengend Snippet: List of commercially available PCR-based assays for detection of Mucorales and Pneumocystis jirovecii .

Article Snippet: AusDiagnostics Pneumonia and Atypical Pneumonia panels , AusDiagnostics Pty Ltd., Mascot, NSW, Australia , Multiplex tandem PCR , Targets unknown Pneumocystis jirovecii , Cryptococcus neoformans species complex, and Aspergillus fumigatus complex.

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction